This circle provides a barrier to liquids such as antibody solutions or chromogenic substrates applied to the sections, thus helping to obtain more uniform immunohistochemical staining and making it possible . This section lists reagents that are not used for a specific instrument. For reagents specifically developed for an instrument, . We offer a range of other products in our Immunohistochemistry section. These products cover chromogenic substrates, blocking reagents, buffers and diluents, counterstains, mounting media, proteolytic enzymes, and Dako Pen , slides and Pascal strips.
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I use Dako pen to draw circles to confine the liquid during immunofluorescence staining. However, I found out it may fall off and stick to my sample, which cause blocking the tissue from all the antibody adde or blocking the view when I take pictures. Dako Pen , Wholesale Various High Quality Dako Pen Products from Global Dako Pen Suppliers and Dako Pen Factory,Importer,Exporter at Alibaba.
PAP pen for drawing hydrophobic barriers on tissue, enabling differential staining of two sections or saving on reagents. Duolink Tips and Tricks (9KB). Use pen to draw a hydrophobic circle around slide-mounted tissue.
Prevents the waste of valuable reagents by keeping liquid pooled in a small droplet. Insoluble in ethanol, acetone and water, but will wash off with xylene.
For use with paraffin-mounted sections or slides treated with albumin or poly-lysine. The size of the pen is 14. The DAKO Pen looks and works almost like a normal felt-tip pen. The pen contains a water -repellent and acetone- and alcohol- insoluble material which is not influenced by . Do not allow sections to dry for the remaining procedure.
Proceed with immunohistochemical staining protocol. After deparaffinization and rehydration of sections, you may continue with the antigen retrieval if affordable (see Sect. .) and . Clear nail polish or Dako Pen (Dako). A slide storage box (Fisher) works quite well for this purpose.
EnVision, HRP-based staining system (Dako). It is not require but this . The sections are encircled by the lipid content from a DAKO pen , limiting the amount of antibody used. The chamber is wrapped in tinfoil from the start when used for alkaline phosphatase experiments. The substrates have to be added in the given order. Буферный раствор может быть приготовлен из таблетированной формы ( БиолоТ, Россия).
Аккуратно промокнуть фильтровальной бумагой предметное стекло вокруг срезов (для образования сухого поля) и обвести срезы гидрофобным фломастером Dako Pen (Dako, Дания), не допуская высыхания . The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet.
The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP. I actually vortex mine to insure goo redistribution. I had wondered about this ( after noticing some very strong solvent odors) and asked them about it, but they said they were acceptable, but that fact changed somewhere along the line.
Ancillaries for IHC cover chromogenic substrates, blocking reagents, buffers and diluents, counterstains, mounting media, proteolytic enzymes, and Dako Pen , slides and Pascal strips.